Recombinant Endoproteinase Lys-C

Catalog Number: RLC18
EC: 3.4.21.50

Storage temperature: -20℃ or below, seal, dark

Source: Recombinant Endoproteinase Lys-C is a genetically engineered protein expressed in E.coli

Protein Sequence:The amino acid sequence of recombinant endoproteinase Lys-C is similar to Lys-C from achromobacterial proteasel

1.DESCRIPTION

This product is a Recombinant Endoproteinase Lys-C, expressed in E. Coli with the similar sequence & proteolytic activity of Lys-C from Achromobacterial proteasel (API). Due to its recombinant form there is no other unwanted serine protease such as Lys-N, Arg-C and only lysine-specific.

2.MAIN FEATURES

Appearance: white or pale white lyophilized powder packed in 0.1mg, 1.0mg & 10mg/vial (according to actual rLys-c content))

Content assay: Lowry Method (labeled ±10%)

SDS-PAGE: Clear single strap, scan purity more than 95%; relative molecular weight is 31±3.1 KDa

RP-HPLC purity: the main peak more than 95%

Enzyme assay activity: ≥ 3.0 AU/mg

Definition of activity: One Amidase Unit (AU) is the amount of enzyme, which produces 1 micromole Ac-Lys-pNA per minute at 25 mmol/L Tris-HCl buffer pH 9.0 at 37°C.

3.RECOMMEND USAGE

Ratio of Lys-C & target protein is 1:1000-1:20000; pH 7.0-9.5, reaction temperature 30-37℃, 2-24 hr.

Optimization by adjusting the temperature, time & enzyme ratio can achieve specific purpose.

Enzyme inhibitors such as TLCK, Guanidine Hydrochloride should not be used

Existence of 0.2% SDS, 1.0% Triton, 6 mol/L Urea, 0.5 mol/L NaCl, 20% Acetonitrile in reaction buffer does not reduce the activity of Lys-C enzymatic digestion.

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: It is lyophilized powder and is stable to use for at least 1 year with storage under -20℃ or below. Reconstituted with double distilled water under 2-8℃ its stability can last for 7 days. It is recommended to dilute the designated concentration by 25mol/L Tris-HCl (pH 8.5). Avoid repeated freeze-thaw after being dissolved.

Stability of transport: The product is stable by blue ice insulation transport.

5.PRODUCT ADVANTAGE

Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.

Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory

requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

6.ANALYSIS of PURITY CONFIRMATION

SDS-PAGE Electrophoresis

RP-HPLC analysis

7.RELATED PRODUCTS

Recombinant carboxypeptidase B

Sequencing grade trypsin

Recombinant trypsin

Recombinant enterokinase